An innovative paper-based device for DNA extraction from processed meat products.

Bibliographic Details
Title: An innovative paper-based device for DNA extraction from processed meat products.
Authors: Batule BS; Department of Chemistry, School of Physics and Chemistry, Gwangju Institute of Science and Technology, 261 Cheomdan-gwagiro, Gwangju 500-712, Republic of Korea., Seok Y; Department of Chemistry, School of Physics and Chemistry, Gwangju Institute of Science and Technology, 261 Cheomdan-gwagiro, Gwangju 500-712, Republic of Korea., Kim MG; Department of Chemistry, School of Physics and Chemistry, Gwangju Institute of Science and Technology, 261 Cheomdan-gwagiro, Gwangju 500-712, Republic of Korea. Electronic address: mkim@gist.ac.kr.
Source: Food chemistry [Food Chem] 2020 Aug 15; Vol. 321, pp. 126708. Date of Electronic Publication: 2020 Mar 30.
Publication Type: Journal Article
Language: English
Journal Info: Publisher: Elsevier Applied Science Publishers Country of Publication: England NLM ID: 7702639 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-7072 (Electronic) Linking ISSN: 03088146 NLM ISO Abbreviation: Food Chem Subsets: MEDLINE
Imprint Name(s): Publication: Barking : Elsevier Applied Science Publishers
Original Publication: Barking, Eng., Applied Science Publishers.
MeSH Terms: Meat Products*, DNA, Mitochondrial/*isolation & purification , Mitochondria/*genetics, Animals ; Cattle ; Chickens/genetics ; DNA, Mitochondrial/genetics ; Sus scrofa/genetics ; Swine
Abstract: Detection of food adulteration is a challenge. However, the identification of adulterated meat in processed products is important for health and personal preference. Mitochondrial genomic DNA (mtDNA) is a good candidate for reliable identification of meat ingredients; however, the extraction of mtDNA from processed products is a bottleneck for development of detection strategies. Therefore, we constructed a rapid (~5 min) mtDNA extraction device. mtDNAs from different meat samples, such as pork (Sus scrofa), chicken (Gallus gallus), and beef (Bos taurus), were successfully detected in up to 0.1% adulterated animal species. We believe that the proposed strategy could be applied to detect animal species from processed meat products to reduce fraudulent practices.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
Contributed Indexing: Keywords: DNA extraction; Dithiothreitol (PubChem CID 446094); Guanidinium thiocyanate (PubChem CID 129830882); Meat detection; Mitochondrial DNA; PCR analysis; Paper chip; Tris hydrochloride (PubChem CID 93573)
Substance Nomenclature: 0 (DNA, Mitochondrial)
Entry Date(s): Date Created: 20200407 Date Completed: 20200707 Latest Revision: 20200707
Update Code: 20210623
DOI: 10.1016/j.foodchem.2020.126708
PMID: 32251924
Published: 2020 Aug 15
Database: MEDLINE

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