An innovative paper-based device for DNA extraction from processed meat products.
|Title:||An innovative paper-based device for DNA extraction from processed meat products.|
|Authors:||Batule BS; Department of Chemistry, School of Physics and Chemistry, Gwangju Institute of Science and Technology, 261 Cheomdan-gwagiro, Gwangju 500-712, Republic of Korea., Seok Y; Department of Chemistry, School of Physics and Chemistry, Gwangju Institute of Science and Technology, 261 Cheomdan-gwagiro, Gwangju 500-712, Republic of Korea., Kim MG; Department of Chemistry, School of Physics and Chemistry, Gwangju Institute of Science and Technology, 261 Cheomdan-gwagiro, Gwangju 500-712, Republic of Korea. Electronic address: email@example.com.|
|Source:||Food chemistry [Food Chem] 2020 Aug 15; Vol. 321, pp. 126708. Date of Electronic Publication: 2020 Mar 30.|
|Publication Type:||Journal Article|
|Journal Info:||Publisher: Elsevier Applied Science Publishers Country of Publication: England NLM ID: 7702639 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-7072 (Electronic) Linking ISSN: 03088146 NLM ISO Abbreviation: Food Chem Subsets: MEDLINE|
|Imprint Name(s):||Publication: Barking : Elsevier Applied Science Publishers
Original Publication: Barking, Eng., Applied Science Publishers.
|MeSH Terms:||Meat Products*, DNA, Mitochondrial/*isolation & purification , Mitochondria/*genetics, Animals ; Cattle ; Chickens/genetics ; DNA, Mitochondrial/genetics ; Sus scrofa/genetics ; Swine|
|Abstract:||Detection of food adulteration is a challenge. However, the identification of adulterated meat in processed products is important for health and personal preference. Mitochondrial genomic DNA (mtDNA) is a good candidate for reliable identification of meat ingredients; however, the extraction of mtDNA from processed products is a bottleneck for development of detection strategies. Therefore, we constructed a rapid (~5 min) mtDNA extraction device. mtDNAs from different meat samples, such as pork (Sus scrofa), chicken (Gallus gallus), and beef (Bos taurus), were successfully detected in up to 0.1% adulterated animal species. We believe that the proposed strategy could be applied to detect animal species from processed meat products to reduce fraudulent practices.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
|Contributed Indexing:||Keywords: DNA extraction; Dithiothreitol (PubChem CID 446094); Guanidinium thiocyanate (PubChem CID 129830882); Meat detection; Mitochondrial DNA; PCR analysis; Paper chip; Tris hydrochloride (PubChem CID 93573)|
|Substance Nomenclature:||0 (DNA, Mitochondrial)|
|Entry Date(s):||Date Created: 20200407 Date Completed: 20200707 Latest Revision: 20200707|
|Published:||2020 Aug 15|